Molecular Ecology Notes (2001) 1, 289-292

Igor V. Sharakhov, Olga Braginets, Charles N. Mbogo and Guiyun Yan (2001)
Isolation and characterization of trinucleotide microsatellites in African malaria mosquito Anopheles funestus
Molecular Ecology Notes 1 (4), 289-292
Abstract: We developed microsatellite markers for an important African malaria mosquito Anopheles funestus Giles. The microsatellite-enriched genomic library was constructed and screened with single-strand oligonucleotides [(CCT)₁₇, (AAT)₁₇, (CAG)₁₇ and (GA)₂₅] as probes. Among the 47 pairs of polymerase chain reaction primers screened, 31 produced successful and consistent amplification. Although only a few A. funestus individuals from one geographical location were used to screen microsatellite marker polymorphism, 27 markers were found polymorphic and four markers monomorphic. Most polymorphic markers are trinucleotide markers. Isolation of polymorphic microsatellite markers provide useful tools for A. funestus population genetic studies and genome mapping.
(The abstract is excluded from the Creative Commons licence and has been copied with permission by the publisher.)
Link to article at publishers website
Database assignments for author(s): Igor V. Sharakhov

Research topic(s) for pests/diseases/weeds:
molecular biology - genes

---

Pest and/or beneficial records:

Beneficial	Pest/Disease/Weed	Crop/Product	Country	Quarant.
	Anopheles funestus