Molecular Ecology Notes (2001) 1, 223-225

Jan E. Conn, Jonathan P. Bollback, David Y. Onyabe, Tessa N. Robinson, Richard C. Wilkerson and Marinete M. Póvoa (2001)
Isolation of polymorphic microsatellite markers from the malaria vector Anopheles darlingi
Molecular Ecology Notes 1 (4), 223-225
Abstract: High molecular weight DNA was extracted from the primary Neotropical malaria vector, Anopheles darlingi from Capanema, Pará, Brazil, to create a small insert genomic library, and then a phagemid library. Enriched sublibraries were constructed from the phagemid library using a microsatellite oligo primed second strand synthesis protocol. The resulting 242 760 individual clones were screened. The mean clone size of the positive clones was 302 bp. Flanking primers were designed for each suitable microsatellite sequence. Eight polymorphic loci were optimized and characterized. The allele size ranges are based on 253 samples of A. darlingi from eastern Amazonian and central Brazil.
(The abstract is excluded from the Creative Commons licence and has been copied with permission by the publisher.)
Link to article at publishers website
Database assignments for author(s): Jan E. Conn, Richard C. Wilkerson

Research topic(s) for pests/diseases/weeds:
molecular biology - genes

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Pest and/or beneficial records:

Beneficial	Pest/Disease/Weed	Crop/Product	Country	Quarant.
	Anopheles darlingi		Brazil (south)
	Anopheles darlingi		Brazil (NE)