Molecular Breeding (1999) 5, 357-365

Julia A. Charity, Marilyn A. Anderson, Dennis J. Bittisnich, Malcolm Whitecross and T.J.V. Higgins (1999)
Transgenic tobacco and peas expressing a proteinase inhibitor from Nicotiana alata have increased insect resistance
Molecular Breeding 5 (4), 357-365
Abstract: Proteinase inhibitors have been used to increase resistance to insect pests in transgenic plants. A cDNA clone encoding a multi-domain proteinase inhibitor precursor from Nicotiana alata (Na-PI) was transferred into tobacco and peas under the control of a promoter from a ribulose-1, 5-bisphosphate carboxylase small subunit gene. The Na-PI precursor was cleaved in the leaves of transgenic tobacco and peas, and M_r 6000 polypeptides accumulated to levels of 0.3% and 0.1%, respectively, of the total soluble protein. The Na-PI cDNA segregated as a dominant Mendelian trait and was stably transmitted for at least two generations of both species. Helicoverpa armigera larvae that ingested tobacco or pea leaves containing Na-PI exhibited higher mortality or were delayed in growth and development relative to control larvae.
(The abstract is excluded from the Creative Commons licence and has been copied with permission by the publisher.)
Link to article at publishers website
Database assignments for author(s): Thomas J.V. Higgins

Research topic(s) for pests/diseases/weeds:
resistance/tolerance/defence of host

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Pest and/or beneficial records:

Beneficial	Pest/Disease/Weed	Crop/Product	Country	Quarant.
	Helicoverpa armigera