Medical and Veterinary Entomology (2003) 17, 316-325

D. Otranto, D.D. Colwell, D. Traversa and J.R. Stevens (2003)
Species identification of Hypoderma affecting domestic and wild ruminants by morphological and molecular characterization
Medical and Veterinary Entomology 17 (3), 316-325
Abstract: Cuticular structures and the sequence of the cytochrome oxidase I gene were compared for Hypoderma bovis (Linnaeus), Hypoderma lineatum (De Villers), Hypoderma actaeon Brauer, Hypoderma diana Brauer and Hypoderma tarandi (Linnaeus) (Diptera, Oestridae). Third-stage larvae of each species were examined by scanning electron microscopy revealing differences among species in the pattern and morphology of spines on the cephalic and thoracic segments, by spine patterns on the tenth abdominal segment, and by morphology of the spiracular plates. The morphological approach was supported by the molecular characterization of the most variable region of the cytochrome oxidase I (COI) gene of these species, which was amplified by polymerase chain reaction and analysed. Amplicons were digested with the unique restriction enzyme, BfaI, providing diagnostic profiles able to simultaneously differentiate all Hypoderma species examined. These findings confirm the utility of morphological characters for differentiating the most common Hypoderma larvae and reconfirm the power of the COI gene for studying insect identification and systematics.
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Link to article at publishers website
Database assignments for author(s): Domenico Otranto, Douglas D. Colwell

Research topic(s) for pests/diseases/weeds:
identification/taxonomy

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Pest and/or beneficial records:

Beneficial	Pest/Disease/Weed	Crop/Product	Country	Quarant.
	Hypoderma (genus)
	Hypoderma lineatum
	Hypoderma bovis
	Hypoderma tarandi
	Hypoderma actaeon
	Hypoderma diana