Letters in Applied Microbiology (2005) 41, 390-396

L. Cavaglieri, J. Orlando and M. Etcheverry (2005)
In vitro influence of bacterial mixtures on Fusarium verticillioides growth and fumonisin B₁ production: effect of seeds treatment on maize root colonization
Letters in Applied Microbiology 41 (5), 390-396
Abstract: Aims: Enterobacter cloacae, Microbacterium oleovorans, Pseudomonas solanacearum and Bacillus subtilis were investigated in order to evaluate: (i) the inoculum size of two bacterial mixtures on Fusarium verticillioides growth and fumonisin B₁ production in vitro at different water activities and (ii) the efficacy of a seed treatment with the best bacterial mixture on F. verticillioides root colonization in greenhouse studies.
Methods and Results: The influence of bacterial mixtures (1 = E. cloacae and M. oleovorans and 2 = P. solanacearum and B. subtilis) to antagonize 13 F. verticillioides strains at different inoculum concentrations (10⁸, 10⁹ and 10¹⁰ cells ml^-1) and water activities (0·937, 0·955 and 0·982 a_W) were examined. Antibiosis, growth rate and fumonisin B₁ production were determined. Bacterial mixture 1 proved to exert the most effective control. Seed treatment with mixture 1 at 10⁸ cells ml^-1 had the best inhibitory effect on F. verticillioides root colonization.
Conclusions: These results suggest that the combination E. cloacae and M. oleovorans has the potential for the biological control of F. verticillioides as a maize seed inoculant.
Significance and Impact of the Study: The application of this knowledge contributes to prevent the vertical transmission of F. verticillioides.
(The abstract is excluded from the Creative Commons licence and has been copied with permission by the publisher.)
Link to article at publishers website
Database assignments for author(s): Lilia Cavaglieri, Miriam G. Etcheverry

Research topic(s) for pests/diseases/weeds:
biocontrol - natural enemies
Research topic(s) for beneficials or antagonists:
formulation/storage of bioagents

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Pest and/or beneficial records:

Beneficial	Pest/Disease/Weed	Crop/Product	Country	Quarant.
	Fusarium verticillioides	Maize/corn (Zea mays)
Enterobacter cloacae (antagonist)	Fusarium verticillioides	Maize/corn (Zea mays)
Microbacterium oleivorans (antagonist)	Fusarium verticillioides	Maize/corn (Zea mays)