Letters in Applied Microbiology (1999) 28, 440-444

M.D. Ferrandis, R. Andrew, M. Porcar, J. Iriarte, V. Cosmao-Dumanoir, M.-M. Lecadet, P. Caballero and J. Ferré (1999)
Characterization of Bacillus thuringiensis serovar bolivia (serotype H63), a novel serovar isolated from the Bolivian high valleys
Letters in Applied Microbiology 28 (6), 440-444
Abstract: The type strain Bacillus thuringiensis var. bolivia (serotype H63), isolated from the Bolivian high valleys, has been characterized at different levels. Its parasporal crystal has an unusual shape and it is composed of a protein of 155 kDa which shows two bands of 75 and 80 kDa after activation. Analysis by PCR shows the presence of cry1 genes, and amplification with specific primers gave products for cry1E, cry1D, cry4A and cry4B with sizes different to those expected. Immunoblotting tests showed positive reaction for Cry1E, Cry3A, Cry4A and Cry11A crystal proteins. The plasmid pattern revealed two large and two small plasmids. Toxicity tests were performed against 14 insects and a slight toxicity was found against Plutella xylotella and Trichoplusia ni.
(The abstract is excluded from the Creative Commons licence and has been copied with permission by the publisher.)
Link to article at publishers website
Database assignments for author(s): Primitivo Caballero, Juan Ferre

Research topic(s) for pests/diseases/weeds:
biocontrol - natural enemies
Research topic(s) for beneficials or antagonists:
general biology - morphology - evolution

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Pest and/or beneficial records:

Beneficial	Pest/Disease/Weed	Crop/Product	Country	Quarant.
Bacillus thuringiensis (entomopathogen)			Bolivia
Bacillus thuringiensis Cry1E-toxin (biocontrol agent)
Bacillus thuringiensis Cry4A-toxin (entomopathogen)