Journal of the American Mosquito Control Association (2012) 28, 119-122

Rebecca T. Trout Fryxell, Stephanie N. Seifert, Yoosook Lee, Adama Sacko, Gregory Lanzaro and Anthony Cornel (2012)
The knockdown resistance mutation and knockdown time in Anopheles gambiae collected from Mali evaluated through a bottle bioassay and a novel insecticide-treated net bioassay
Journal of the American Mosquito Control Association 28 (2), 119-122
Abstract: Successful malaria management in Mali includes the use of pyrethroids and insecticide-treated nets (ITNs) for mosquito control; however, management is threatened by the spread of insecticide resistance detected via the knockdown resistance (kdr) allele. In a preliminary study, we compared the knockdown times of Anopheles gambiae from Mali using a novel ITN bioassay and the World Health Organization (WHO) bottle bioassay. Additionally, the frequency and relationship between kdr genotypes, molecular forms, and pyrethroid resistance were analyzed. The S molecular form was predominant and accounted for 76% of the assayed population. Both kdr resistant alleles, West Africa resistant (kdr-w) and East Africa resistant (kdr-e), were observed. There was no significant difference in knockdown time based on kdr genotype or molecular form of individual mosquitoes, but mosquitoes in the ITN bioassay homozygous for the kdr-w allele were knocked down significantly faster than those in the WHO bottle bioassay. The ITN bioassay provides an additional indicator of insecticide efficacy because ITNs, frequently used within homes, are the most common form of vector control and malaria prevention, and the ITN bioassays can evaluate seasonal field effects.
(The abstract is excluded from the Creative Commons licence and has been copied with permission by the publisher.)
Link to article at publishers website
Database assignments for author(s): Rebecca Trout Fryxell, Gregory C. Lanzaro, Yoosook Lee

Research topic(s) for pests/diseases/weeds:
pesticide resistance of pest

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Pest and/or beneficial records:

Beneficial	Pest/Disease/Weed	Crop/Product	Country	Quarant.
	Anopheles gambiae		Mali