Journal of Virology (2001) 75, 5385-5390

Yasushi Okinaka, Kazuyuki Mise, Eri Suzuki, Tetsuro Okuno and Iwao Furusawa (2001)
The C terminus of Brome mosaic virus coat protein controls viral cell-to-cell and long-distance movement
Journal of Virology 75 (11), 5385-5390
Abstract: To investigate the functional domains of the coat protein (CP; 189 amino acids) of Brome mosaic virus, a plant RNA virus, 19 alanine-scanning mutants were constructed and tested for their infectivity in barley and Nicotiana benthamiana. Despite its apparent normal replicative competence and CP production, the C-terminal mutant F184A produced no virions. Furthermore, virion-forming C-terminal mutants P178A and D182A failed to move from cell to cell in both plant species, and mutants D181A and V187A showed host-specific movement. These results indicate that the C-terminal region of CP plays some important roles in virus movement and encapsidation. The specificity of certain mutations for viral movement in two different plant species is evidence for the involvement of host-specific factors.
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Database assignments for author(s): Kazuyuki Mise

Research topic(s) for pests/diseases/weeds:
molecular biology - genes

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Pest and/or beneficial records:

Beneficial	Pest/Disease/Weed	Crop/Product	Country	Quarant.
	Brome mosaic virus