Journal of Virology (1977) 24, 412-415

A.M. Crawford and J. Kalmakoff (1977)
Effect of alkaline protease on the antigenic nature of Wiseana nuclear polyhedrosis virus polyhedron protein
Journal of Virology 24 (1), 412-415
Abstract: Polyhedron protein from Wiseana spp. nuclear polyhedrosis virus was found to be degraded by an alkali protease when polyhedra are dissolved in alkali. The protease activity did not occur at high pH (0.1 M NaOH) and was inactivated by heating polyhedra to 70 degrees C for 3 h. The products from the protease degradation of Wiseana spp. nuclear polyhedrosis virus polyhedron protein retain the antigenicity of undegraded polyhedron protein as measured by the direct solid-phase radioimmunoassay and immunoadsorption. Degradation products below 27,000 daltons could not be detected by the sandwich radioimmunoassay, indicating that they are probably monovalent.
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(original language: English)
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Database assignments for author(s): Allan M. Crawford

Research topic(s) for pests/diseases/weeds:
biocontrol - natural enemies
Research topic(s) for beneficials or antagonists:
molecular biology - genes

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Pest and/or beneficial records:

Beneficial	Pest/Disease/Weed	Crop/Product	Country	Quarant.
Alphabaculovirus wisignatae (entomopathogen)
Alphabaculovirus eppostvittanae (entomopathogen)