Journal of Vector Ecology (2007) 32, 123-133

Josiane Etang, Lucien Manga, Jean-Claude Toto, Pierre Guillet, Etienne Fondjo and Fabrice Chandre (2007)
Spectrum of metabolic-based resistance to DDT and pyrethroids in Anopheles gambiae s.l. populations from Cameroon
Journal of Vector Ecology 32 (1), 123-133
Abstract: Some populations of Anopheles gambiae s.l. from Cameroon were reported to develop resistance to DDT or pyrethroids but were free of the kdr mutation 'Leucine-Phenylalanine' (Leu-Phe). This study reports on the metabolic activity of non-specific esterases (NSEs), mixed function oxidases (MFOs), and glutathione S-transferases (GSTs), three enzyme systems commonly involved in insecticide resistance. Biochemical assays were performed in DDT or pyrethroidresistant populations of An. gambiae s.l. from Douala, Mbalmayo, Pitoa, and Simatou neighborhoods. Enzyme activity was compared to the Kisumu-susceptible reference strain using the Mann-Whitney test. Most of the tested samples had elevated NSE activity (P<0.02). The Douala sample evenly displayed elevated GST activity (P<0.001), while high MFO level was recorded in the Pitoa sample (P<0.001). MFO or GST levels were sometimes lower or similar to that of the Kisumu strain. These results suggest metabolic detoxification is a major DDT or pyrethroid resistance mechanism and emphasize the need for further investigations on An. gambiae s.l. resistance mechanisms in Cameroon.
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Database assignments for author(s): Fabrice Chandre, Josiane Etang, Pierre Guillet

Research topic(s) for pests/diseases/weeds:
pesticide resistance of pest

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Pest and/or beneficial records:

Beneficial	Pest/Disease/Weed	Crop/Product	Country	Quarant.
	Anopheles gambiae		Cameroon