Journal of Plant Protection Research (2013) 53, 195-202

Bita Valizadeh, Arash Zibaee and Jalal Jalali Sendi (2013)
Inhibition of digestive α-amylases from Chilo suppressalis Walker (Lepidoptera: Crambidae) by a proteinaceous extract of Citrullus colocynthis L. (Cucurbitaceae)
Journal of Plant Protection Research 53 (3), 195-202
Abstract: The striped rice-stem borer, Chilo suppressalis (Walker) is one of the most important pest of rice worldwide. In this study, a proteinaceous extract from Citrullus colocynthis L. shows various degrees of inhibition to digestive α-amylases of C. suppressalis. Digestive α-amylases of larvae were inhibited by different concentrations (approximately 50%) of C. colocynthis amylase inhibitor (CCAI). One of the isozymes totally disappeared and the sharpness of another decreased on native-PAGE electrophoresis. The pH dependency of inhibition revealed that the enzyme was inhibited in a wide range of pH from 7-10, and the optimal pH of the enzyme occurred in lepidopteran larvae. The highest inhibition of α-amylase by CCAI was observed at 25°C; the temperature near the optimal temperature of amylolytic activity. A time-course experiment demonstrated that enzymatic activity was the highest, 30 min after the onset of the experiment, when the highest inhibition occurred. The enzyme kinetic studies using Lineweaver-Burk analysis, revealed a mixed inhibition of CCAI on α-amylase activity. The current study is the first basic one using α-amylase inhibitor against C. suppressalis. This study opens the way for transgenic rice varieties containing inhibitors.
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Database assignments for author(s): Arash Zibaee, Jalal Jalali Sendi

Research topic(s) for pests/diseases/weeds:
control - general

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Pest and/or beneficial records:

Beneficial	Pest/Disease/Weed	Crop/Product	Country	Quarant.
	Chilo suppressalis	Rice (Oryza)	Iran