Journal of Plant Protection Research (2010) 50, 133-139

Bhagwan Singh Sanodiya, Gulab Singh Thakur, Rakesh Kumar Baghel, Akhilesh Kumar Pandey, Godavarthi Bhogendra Krishna Satya Prasad and Prakash Singh Bisen (2010)
Isolation and characterization of tenuazonic acid produced by Alternaria alternata, a potential bioherbicidal agent for control of Lantana camara
Journal of Plant Protection Research 50 (2), 133-139
Abstract: Microbial preparation of herbicide is defined as bioherbicide that can control the weed. In this approach, indigenous plant pathogens isolated from weeds are cultured to produce the large numbers of infective propagules which are applied at a rate that will cause high levels of infection leading to suppression of the target weed. During the present investigation, cell free culture filtrate (CFCF) of Alternaria alternata was evaluated for its phytotoxicity against a noxious weed Lantana camara. The results of cut shoot, seedling and detached leaf bioassays revealed the presence of a toxic metabolite in the CFCF and a significant reduction in chlorophyll and protein content were also noticed. Phytotoxic moiety was further purified and characterized by using solvent partition, thinlayer chromatography (TLC), FTIR and ¹H NMR analysis. The acetone extract induced maximum phytotoxic damage at a concentration of 100 µg/ml and TLC purified fraction also exhibited herbicidal potential. The toxic compound was identified as tenuazonic acid upon comparison with FTIR and ¹HNMR spectra. This is the first evidence that confirmed the herbicidal potentialof a biorational, tenuazonic acid was produced by submerged fermentation of A.alternata.
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Database assignments for author(s): Akhilesh Kumar Pandey

Research topic(s) for pests/diseases/weeds:
biocontrol - natural enemies
Research topic(s) for beneficials or antagonists:
molecular biology - genes

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Pest and/or beneficial records:

Beneficial	Pest/Disease/Weed	Crop/Product	Country	Quarant.
	Lantana camara (weed)		India
Alternaria alternata (weed pathogen)	Lantana camara (weed)		India