Journal of Plant Pathology (2017) 99, 361-369

K. Kalaiponmani, R. Thangavelu and G. Varun (2017)
Optimization of protein isolation and preliminary comparative proteomics of pathogenic Fusarium oxysporum f. sp. cubense (P-Foc) and non-pathogenic Fusarium oxysporum (np-Fo)
Journal of Plant Pathology 99 (2), 361-369
Abstract: Panama wilt of banana caused by F. oxysporum f. sp. cubense (Foc) is a devastating disease of banana and in severe condition causes 100% yield loss. In order to understand the molecular mechanism of pathogenicity for the effective management of the wilt disease, three protocols (MgCl2/CHAPS, TCA-Acetone and TCA/Phenol-SDS) were evaluated to extract protein from Foc mycelial tissue. Comparatively, TCA/Phenol-SDS method of protein precipitation and extraction resulted in highest protein quantity (0.76 ± 0.025 mg/g of mycelial tissue) and ca. 600 spots were identified in Coomassie Brilliant Blue (CBB) stained gels. A comparative proteomic study conducted on pathogenic F. oxysporum f. sp. cubense (P-Foc) (race 1, VCG 0124) and non-pathogenic Fusarium oxysporum (np-Fo) revealed a difference at the proteome level. In the comparative proteomics study, 23 spots were found to be differentially expressed with > 1.5 fold intensity differences between P-Foc and np-Fo. Preliminarily, six spots (five significantly upregulated and one unique protein) from P-Foc were randomly picked and subjected to MALDI-TOF MS analysis. Further, MASCOT search and functional annotation revealed that these upregulated proteins identified in P-Foc may be involved in invasive growth, signal transduction and pathogen persistence during adverse condition.
(The abstract is excluded from the Creative Commons licence and has been copied with permission by the publisher.)
Link to article at publishers website
Database assignments for author(s): Raman Thangavelu

Research topic(s) for pests/diseases/weeds:
molecular biology - genes

---

Pest and/or beneficial records:

Beneficial	Pest/Disease/Weed	Crop/Product	Country	Quarant.
	Panama disease of banana	Banana/plantain (Musa)	India