Journal of Plant Pathology (2008) 90, 375-381

M. Zaccardelli, F. Campanile, C. Moretti and R. Buonaurio (2008)
Characterization of Italian populations of Xanthomonas campestris pv. campestris using primers based on DNA repetitive sequences
Journal of Plant Pathology 90 (2), 375-381
Abstract: One-hundred and forty-one strains of Xanthomonas campestris pv. campestris (Xcc), the causal agent of vascular blackening of crucifers were isolated from different crucifer plants in central and southern Italian regions and characterized for DNA polymorphism by PCR, using primers based on repetitive sequences. By M13-PCR, all strains, except for two, were distributed in three main clusters: a, with similarity index (SI) 0.78, containing 70 strains grouped in 19 haplotypes; b (SI = 0.80) containing 54 strains in 17 haplotypes; g (SI = 0.75) containing 15 strains in 6 haplotypes. By BOX-PCR, all strains were distributed in three main clusters: a (SI = 0.58) containing 102 strains distributed in 9 haplotypes; b (SI = 0.45), including 24 strains distributed in 9 haplotypes; g (SI = 0.37), including 15 strains distributed in 3 haplotypes. A very large variability was observed in these Italian populations of Xcc especially using M13-PCR. No obvious relationships were observed between the strains comprised in each cluster, sub-cluster or haplotype and their geographic origin or host plant.
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Database assignments for author(s): Massimo Zaccardelli, Chiaraluce Moretti, Roberto Buonaurio

Research topic(s) for pests/diseases/weeds:
molecular biology - genes

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Pest and/or beneficial records:

Beneficial	Pest/Disease/Weed	Crop/Product	Country	Quarant.
	Xanthomonas campestris pv. campestris	Cabbage (Brassica oleracea)	Italy
	Xanthomonas campestris pv. campestris	Brassica - other species	Italy
	Xanthomonas campestris pv. campestris	Crambe (genus)	Italy
	Xanthomonas campestris