Journal of Plant Pathology (2007) 89, 141-147

M. Gullusci and M. Turina (2007)
Silencing of cryparin, a cell wall hydrophobin, in Cryphonectria parasitica
Journal of Plant Pathology 89 (1), 141-147
Abstract: Cryparin is a cell wall hydrophobin abundantly expressed in Cryphonectria parasitica, the ascomycete fungus causing chestnut blight. In this study we compare the cryparin-minus phenotype of a previously characterized knockout strain to that of silenced strains obtained by the transformation of the fungus with a strong elicitor of post-transcriptional gene silencing. We show that cryparin expression can be silenced very efficiently and that the knockout phenotype is similar to the phenotypes obtained through gene silencing. Silenced strains were also experimentally infected with Cryphonectria hypovirus 1 (CHV-1) with no obvious effect on cryparin expression. We also tested the possibility that p29, a protease expressed by the CHV-1 genome, might act as a suppressor of gene silencing in plants.
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Database assignments for author(s): Massimo Turina

Research topic(s) for pests/diseases/weeds:
molecular biology - genes
biocontrol - natural enemies
Research topic(s) for beneficials or antagonists:
molecular biology - genes

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Pest and/or beneficial records:

Beneficial	Pest/Disease/Weed	Crop/Product	Country	Quarant.
	Cryphonectria parasitica
Cryphonectria parasitica hypoviruses (antagonist)	Cryphonectria parasitica