Journal of Plant Pathology (2006) 88, p. S52 (Parrella et al.)

G. Parrella, A. De Stradis and C. Vovlas (2006)
High incidence of brigth yellow mosaic associated with mixed infections of Artichoke latent virus and an isometric virus in globe artichoke in Campania
Journal of Plant Pathology 88 (3, Special Issue), S52-S52
S.I.Pa.V XIII National Meeting - Foggia, 12-16 September 2006 - Poster
Abstract: In 2005, a virus-like disease was observed in commercial globe artichoke fields in Campania (Southern Italy). Symptoms consisted of stunting and discoloration of the leaf blade (yellow blotches, rings and line patterns). Disease incidence was around 50-60% with peaks of almost 100% in some areas and was present again in 2006, with a similar apperance and incidence. To identify the possible causal agents, symptomatic plant samples were collected from different locations (Scafati, Pompei). Observations of leaf-dip preparations by electron microscopy showed the presence of a filamentous virus ca. 730-740 nm long and of an isometric virus ca. 30 nm in diameter. Ultrathin sections of Nicotiana benthamiana tissues inoculated with leaf extracs from symptomatic artichokes showed typical inclusion bodies caused by potyviruses. Based on the aspect of cylindrical inclusions (CI) the potyvirus was assigned to the subdivision-II, which induces type-2 CIs, consisting of pinwheels and more or less straight laminate aggregates. The filamentous virus was clearly decorated by an antiserum to Artichoke latent virus (ArLV). Isometric virus particles were readily recognized in ultrathin sections, especially when they were arranged to form circular arrays identical to those typically induced by Broad bean wilt virus (BBWB). Attempts to decorate the isometric particles with antisera against the subgroup B of nepoviruses failed, but no serological identification of BBWV was made. The outbreaks of bright yellow mosaic of globe artichoke observed in Campania, caused by mixed infections of Ar-LV and putative BBWV resemble those reported earlier from Apulia, a neighbouring region.
Database assignments for author(s): Giuseppe Parrella, Angelo De Stradis

Research topic(s) for pests/diseases/weeds:
general biology - morphology - evolution

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Pest and/or beneficial records:

Beneficial	Pest/Disease/Weed	Crop/Product	Country	Quarant.
	Artichoke latent virus	Artichoke (Cynara)	Italy