Journal of Plant Pathology (2006) 88, p. S49 (Marra et al.)

R. Marra, P. Ambrosino, V. Carbone, F. Vinale, S.L. Woo, M. Ruocco, R. Ciliento, S. Lanzuise, S. Ferraioli, I. Soriente, D. Turrà, V. Fogliano, F. Scala and M. Lorito (2006)
Proteomic analysis of the complex three-way interaction occurring between plant, fungal pathogens and Trichoderma atroviride strain P1
Journal of Plant Pathology 88 (3, Special Issue), S49-S49
S.I.Pa.V XIII National Meeting - Foggia, 12-16 September 2006 - Poster
Abstract: We have used a proteomic approach to analyse the molecular factors involved in the complex three-way interaction occurring between plant (bean), fungal pathogens (Botrytis cinerea or Rhizoctonia solani) and the antagonist Trichoderma atroviride strain P1. Interactions were studied on water agar plates, where the fungal mycelia, grown separately on cellophane, were layered over plant tissues (roots or leaves). Proteomes were obtained individually from each partner and separated by 2-D gel electrophoresis. Differential proteins were partially identified using tryptic digestion, MALDI-TOF mass spectrometry and in silico analysis. In the plant proteome, specific pathogenesis-related proteins and other disease-related factors (i.e. potential resistance genes) were found to accumulate in the presence of the antagonist alone or together with the fungal pathogens. In the B. cinerea proteome, an overall view of the proteins involved in the infection process was obtained and many of the proteins could be identified (i.e. cyclophilins, superoxide dismutase, cutinase, etc.). Many differential proteins obtained from the proteome of T. atroviride showed interesting homologies with fungal hydrophobins, ABC transporters, stress-related proteins (heat shock proteins), chitin synthase, Hex1 proteins, etc. For example, a fungal hydrophobin accumulated by T. atroviride strain P1 in the presence of R. solani showed homology to the NIP1 avirulence factor. This finding supports the hypothesis that the molecular cross-talk between the plant and Trichoderma involves a gene-for-gene (avr-R) interaction.
Database assignments for author(s): Sheridan L. Woo, Francesco Vinale, Roberta Marra, Michelina Ruocco, Felice Scala

Research topic(s) for pests/diseases/weeds:
biocontrol - natural enemies
molecular biology - genes
Research topic(s) for beneficials or antagonists:
molecular biology - genes

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Pest and/or beneficial records:

Beneficial	Pest/Disease/Weed	Crop/Product	Country	Quarant.
	Botrytis cinerea
	Rhizoctonia solani
Trichoderma atroviride (antagonist)	Botrytis cinerea
Trichoderma atroviride (antagonist)	Rhizoctonia solani