Journal of Plant Pathology (2006) 88, p. S38 (Ciliento et al.)

R. Ciliento, S.L. Woo, P. Marinelli, B. Navarra, M. Siena, R. Marra, F. Vinale, S. Ferraioli, P. Ambrosino, I. Soriente, M. Ruocco, D. Turrà, S. Lanzuise, M.A. Obregon Gomez and M. Lorito (2006)
Physiological, biochemical and biological characterization of novel Trichoderma strains widely applied in Costa Rica
Journal of Plant Pathology 88 (3, Special Issue), S38-S38
S.I.Pa.V XIII National Meeting - Foggia, 12-16 September 2006 - Poster
Abstract: Trichoderma spp. are cosmopolitan in all soil types and frequently represent one of the main component of the soil microflora. This is attributed to their diverse biological activities, aggressiveness and competitive nature. We characterized physiologically, biochemically and biologically seven Trichoderma strains isolated in Costa Rica and widely used in Central America as biopesticides. In all tests, the strains were grown on PDA or minimal medium containing sucrose as the only carbon source, at temperatures of 25° or 30°C in comparison with the strain P1 of T. atroviride. We measured mycelial growth, sporulation time and quantity of conidia produced. Strains Bulgvir, Harz, TF, PB17 grew faster and with more spores productions than P1 when grown at 30°C. Biochemical analysis indicated that TF and PB17 in comparison to P1 accumulated in culture filtrates higher endoand exo-chitinase and similar glucanase and xylanase activity. In vitro confrontation assays were performed against the pathogens Rhizoctonia solani and Botrytis cinerea. Strains Bulgvir, Harz, Exc, TF, PB17 were able to control both pathogens more effectively than P1. In summary, our results demonstrate that the tested strains from Costa Rica exhibited a much higher fitness, enzyme production and antifungal activity, especially when grown at temperature of 30°C instead of 25°C in comparison to the well-known strain P1 of T. atroviride. Species identification, proteome analysis and application of these strains as biopesticides in a mediterranean climate zone are being carried out.
Database assignments for author(s): Sheridan L. Woo, Francesco Vinale, Roberta Marra, Michelina Ruocco

Research topic(s) for pests/diseases/weeds:
biocontrol - natural enemies
Research topic(s) for beneficials or antagonists:
general biology - morphology - evolution

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Pest and/or beneficial records:

Beneficial	Pest/Disease/Weed	Crop/Product	Country	Quarant.
	Botrytis cinerea
	Rhizoctonia solani
Trichoderma (anamorphic genus - antagonists)			Costa Rica