Journal of Plant Pathology (2005) 87, p. 299 (Moretti et al.)
C. Moretti, M. Scortichini and R. Buonaurio (2005)
Preliminary results of cloning an AraC gene from Pseudomonas avellanae
Journal of Plant Pathology 87 (4), 299-299
XII Meeting, Italian Society for Plant Pathology, Reggio Calabria, 29/9 - 1/10, 2005 (poster)
Abstract: In the past decades, severe epidemics of bacterial canker and decline of hazelnut (Corylus avellana L.), caused by Pseudomonas avellanae (Psallidas) Janse et al., have been reported in northern Greece and central Italy. Greek and Italian strains of the bacterium belong to two separate populations, which can be discriminated by monoclonal antibodies raised against the cell wall polysaccharides and by rep-PCR analysis, using ERIC primer sets. To further characterize at the molecular level the two populations of the bacterium, a specific 700 bp fragment of the Greek strains (BPIC 631), obtained with ERIC-PCR, was cloned in the pCR®4-TOPO vector and sequenced. Its deduced aminoacidic sequence, compared with those from GenBank, showed high similarity with proteins of the AraC-XylS family, which are positive transcriptional regulators involved in the control of many important processes related to sugar catabolism, responses to stress, and pathogenesis. In addition, this sequence showed the characteristic helix-turn-helix motif of the AraC-XylS family in the N-terminal region, when analysed by PROSITE, a database of protein families and domains. Experiments are in progress to clone the complete araC gene of P. avellanae and to verify if it is present in both populations of the bacterium.
Database assignments for author(s): Marco Scortichini, Chiaraluce Moretti, Roberto Buonaurio
Research topic(s) for pests/diseases/weeds:
molecular biology - genes
Pest and/or beneficial records:
Beneficial | Pest/Disease/Weed | Crop/Product | Country | Quarant.
|
---|---|---|---|---|
Pseudomonas avellanae | Hazelnut (Corylus) | Greece | ||
Pseudomonas avellanae | Hazelnut (Corylus) | Italy |