Journal of Plant Pathology (2005) 87, p. 298 (Marra et al.)

R. Marra, V. Cotarelli, P. Scisciola, P. Ambrosino, M. Ruocco, F. Scala, S.L. Woo and M. Lorito (2005)
Identification of the molecular factors involved in the interactions among the biocontrol agent Trichoderma atroviride, plants and fungal pathogens by a proteomic approach
Journal of Plant Pathology 87 (4), 298-298
XII Meeting, Italian Society for Plant Pathology, Reggio Calabria, 29/9 - 1/10, 2005 (poster)
Abstract: Biocontrol agents, such as Trichoderma spp., may influence the fate of plant-pathogen interactions by inducing defence mechanisms in plant and limiting directly or indirectly the pathogen attack. In this work we performed bioassays to analyse the differences of proteomes of bean plants interacting with the pathogens Botrytis cinerea and Rhizoctonia solani, and with the antagonist T. atroviride strain P1. Proteins extracted from the antagonist, the pathogen, and the host during their interactions between two, or among three components, were separated using 2D-gel electrophoresis. Many differential proteins, potentially involved in the interactions, were identified using the PD-QUEST software and characterised via MALDI-TOF MS and in silico analysis. Results can be summarised as follows: (i) in bean plant proteome different defence- and pathogenesis-related proteins were induced by the antagonist and the two pathogens; (ii) in Trichoderma proteome, besides the metabolic and stress-related proteins, an ABC transporter, different cyclophilins and a hydrophobin were differentially expressed as compared to the control; (iii) in B. cinerea proteome virulence factors and defence proteins accumulated. Data obtained demonstrate the usefulness of this approach for the identification of molecular determinants involved in the three-component interactions. This study is, to our knowledge, the first report that analyses the complex factors that play a role during the cross-talk between plants, fungal pathogens and antagonistic fungi.
Database assignments for author(s): Sheridan L. Woo, Roberta Marra, Michelina Ruocco, Felice Scala

Research topic(s) for pests/diseases/weeds:
biocontrol - natural enemies
Research topic(s) for beneficials or antagonists:
general biology - morphology - evolution
molecular biology - genes

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Pest and/or beneficial records:

Beneficial	Pest/Disease/Weed	Crop/Product	Country	Quarant.
	Botrytis cinerea
	Rhizoctonia solani
Trichoderma atroviride (antagonist)	Botrytis cinerea
Trichoderma atroviride (antagonist)	Rhizoctonia solani