Journal of Plant Pathology (2005) 87, p. 292 (Comes et al.)

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S. Comes, A. Fanigliulo, R. Pacella, G. Parrella and A. Crescenzi (2005)
Characterization of a Potato virus Y isolate from Capsicum chinense cv Red Habanero
Journal of Plant Pathology 87 (4), 292-292
XII Meeting, Italian Society for Plant Pathology, Reggio Calabria, 29/9 - 1/10, 2005 (poster)
Abstract: Plants of chili pepper, Capsicum chinense Jacquin cv Red Habanero, with leaf mosaic, necrotic rings on the fruits, and stem necrosis were observed in June 2003 in a private garden of Naples (southern Italy). Preliminary serological characterisation showed that these symptoms were associated with the presence of Potato virus Y (PVY). This virus was isolated in Nicotiana tabacum cv Xanthi NC and characterised biologically and molecularly in its coat protein (CP) gene. Symptoms produced in indicators were generally consistent with those described for PVY, i.e leaf deformation and veinal necrosis in N. occidentalis, mosaic in N. tabacun cv Xanthi and Capsicum annuum, mosaic and epinasty in Lycopersicon esculentum. By contrast, the virus induced local symptoms of chlorotic mottling in Chenopodium spp.. When the viral isolate was exposed to monoclonal antibodies (Mabs) specific to PVYN, PVYC and PVYO, it reacted only with PVYC Mabs. RT-PCR was done on viral RNA extracted from purified virions using a couple of primers specifically designed on the CP coding region of PVYNP (non potato) isolates. PCR product was cloned in a pCRII-TOPO vector using TOPO-TA cloning kit (Invitrogen) and sequenced. Both similarity and phylogenetic analysis made on the 801 bp long coat CP gene sequence showed that the isolate under study is more closely related to other PVY non potato isolates than to those of the PVYO and PVYN group. The highest percentage of similarity was detected with PVY isolates from Solanum nigrum (PVY-SON41), Capsicum annuum (PVY-NNP) and Lycopersicon esculentum (PVY-LYE84.2).
Database assignments for author(s): Aniello Crescenzi, Giuseppe Parrella

Research topic(s) for pests/diseases/weeds:
molecular biology - genes
general biology - morphology - evolution

Pest and/or beneficial records:

Beneficial Pest/Disease/Weed Crop/Product Country Quarant.


Potato virus Y Green pepper/chilli (Capsicum) Italy
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