Journal of Plant Pathology (2005) 87, p. 278 (Gigante et al.)

S. Gigante, M. Ruocco, S. Lanzuise, C. Anacleria, F. Scala, S.L. Woo and M. Lorito (2005)
Use of targeted disruption and GFP mutants to study the role of the gene coding for an ABC transporter in Trichoderma atroviride
Journal of Plant Pathology 87 (4), 278-278
XII Meeting, Italian Society for Plant Pathology, Reggio Calabria, 29/9 - 1/10, 2005
Abstract: The role of the ABC transporter proteins in Trichoderma is still unknown. This work is a preliminary study on the functions of the TABC2 gene of T. atroviride through the targeted gene disruption and the GFP expression under the promoter region of the gene. Hygromycin resistant progeny have been selected and analysed to verify the disruption of the gene and the GFP expression. Disruptant and GFP mutants were tested by molecular analysis (PCR, Southern and northern blotting) to verify the correctness of transformation events. In vitro assays were done to determine the role of the gene during fungal growth and biocontrol activity. The disruptants were grown on PDA to which four different carbon sources (glucose 1%, glycerol 1%, colloidal chitin 0,05% and fructose 1%) were added. As a result, we observed a better growth of the transformants on fructose, while by comparison with the wild type strain, the growth was slow and difficult on glucose and chitin. This was confirmed when all the strains were grown in the presence of culture filtrates of Botrytis cinerea, Rhizoctonia solani, and Pythium ultimum added to SM containing glucose or fructose. The presence of the culture filtrates was not discriminating, while the transformants showed a good rate of growth on fructose and a difficult growth on glucose, as in the previous assay. Finally, we tested all transformant strains for the production of some enzymes. They all showed a lower concentration of all enzymes tested except for b-1,3-glucanase, in comparison with the wild type strain. The results obtained with the disruptants were confirmed by the use of GFP mutants.
Database assignments for author(s): Sheridan L. Woo, Michelina Ruocco, Felice Scala

Research topic(s) for pests/diseases/weeds:
biocontrol - natural enemies
Research topic(s) for beneficials or antagonists:
molecular biology - genes

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Pest and/or beneficial records:

Beneficial	Pest/Disease/Weed	Crop/Product	Country	Quarant.
Trichoderma atroviride (antagonist)