Journal of Plant Pathology (2005) 87, p. 275 (Ambrosino et al.)

P. Ambrosino, R. Marra, V. Carbone, M. Ruocco, S.L. Woo, F. Scala and M. Lorito (2005)
Proteomic analysis of Trichoderma harzianum secreted proteins by peptide mass fingerprint and expressed sequence tag approaches
Journal of Plant Pathology 87 (4), 275-275
XII Meeting, Italian Society for Plant Pathology, Reggio Calabria, 29/9 - 1/10, 2005
Abstract: Trichoderma harzianum is an antagonist able to parasitize phytopathogenic fungi. It secretes proteins that play a pivotal role in the penetration of the host fungus cell wall and in the induction of systemic disease resistance in plant. Two-dimensional gel electrophoresis was used to display the secretome of T. harzianum T22 grown on media containing different inducers of mycoparasitic activities. Twenty-eight differentially expressed proteins were identified by using MALDI-TOF and MS/MS analyses and by searching the Expressed Sequence Tag (EST) database of Trichoderma spp.. Identified up-regulated proteins include: (i) different cell wall-degrading enzymes, such as endo-polygalacturonases, chitin deacetylases, xylanases, cellulases, proteases etc; (ii) proteins with conserved domains involved in pathogen-host interactions such as Ras or LRRs, and (iii) ABC transporter proteins required to regulate the efflux/afflux of molecules across the cell membrane. Water-soluble fractions of extracellular proteins inhibiting tobacco infection by Botrytis cinerea include an endopolylagacturonase and a chitin deacetylase. Future work will address cloning of the genes coding for these two proteins, their disruption and overexpression in Trichoderma, and their expression in vectors to obtain large quantities of proteins.
Database assignments for author(s): Sheridan L. Woo, Roberta Marra, Michelina Ruocco, Felice Scala

Research topic(s) for pests/diseases/weeds:
biocontrol - natural enemies
Research topic(s) for beneficials or antagonists:
molecular biology - genes

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Pest and/or beneficial records:

Beneficial	Pest/Disease/Weed	Crop/Product	Country	Quarant.
	Botrytis cinerea
Trichoderma harzianum (antagonist)	Botrytis cinerea