Journal of Plant Pathology (2004) 86, p. 327 (Palmano et al.)

S. Palmano, R. Lenzi, M. Ciuffo, L. Mela, E. Derin and M. Turina (2004)
Four new potyvirus species in Ranunculus: biology and characterization
Journal of Plant Pathology 86 (4), 327-327
XI Meeting, Italian Society for Plant Pathology, Milan, 29/9 - 1/10, 2004 (poster)
Abstract: Plants of Ranunculus asiaticus with mosaic symptoms and growth abnormalities were collected in Liguria (Italian Riviera, Imperia). Observations by immunoelectron microscopy and molecular tests were carried out. This analysis allowed characterizing four new species of potyviruses previously unidentified in Ranunculus spp.. The amplified DNA fragment coding for the viral capsid protein of the new isolates, obtained by RT-PCR, were sequenced and aligned with the most closely related sequences present in databases. This has allowed to underline the phylogenetic differences of the four potyvirus: three of these viruses are sufficiently distant to suggest that they are new viral species; one of the isolates shows some degree of relatedness with Clover yellow vein virus, first reported from Trifolium. Phylogenetic distance of each virus from other representatives of the family Potyviridae was evaluated by the software Phylip 3.5. The four new virus isolates were multiplied and purified to obtain specific polyclonal antisera. These antisera showed a high specificity against the homologous virus and no reaction with the other potyviruses tested or with healthy controls, in ACP and DAS-ELISA. The same specificity of reaction was confirmed by Western Blot of healthy and infected leaf extracts. Different plants were submitted to mechanical inoculation for biological characterization of the four new potyviruses,. The transmission of these four potyviruses by aphids was also tested in a controlled experiment with Myzus persicae.
Database assignments for author(s): Sabrina Palmano, Massimo Turina

Research topic(s) for pests/diseases/weeds:
general biology - morphology - evolution

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Pest and/or beneficial records:

Beneficial	Pest/Disease/Weed	Crop/Product	Country	Quarant.
	Ranunculus (weeds)
	Potyvirus (genus)