Journal of Plant Pathology (2004) 86, p. 307 (Ambrosino et al.)
P. Ambrosino, V. Scala, R. Marra, F. Vinale, I. Soriente, S. Ferraioli, V. Carbone, M. Ruocco, S.L. Woo and M. Lorito (2004)
Extracellular proteome of Trichoderma harzianum to identify proteins with biotechnological value
Journal of Plant Pathology 86 (4), 307-307
XI Meeting, Italian Society for Plant Pathology, Milan, 29/9 - 1/10, 2004 (poster)
Abstract: Trichoderma harzianum strain T22 parasitizes and controls many phytopathogenic fungi. Commercially, Trichoderma spp. are marketed as biocontrol agents and soil amendments, reducing the application of xenobiotic compounds in the environment and residues in food products. We used a proteomic approach to investigate changes in the complex mixture of cellular proteins of T. harzianum strain T22, obtained under carbon and nitrogen starvation and from in vitro and in vivo interactions with tomato and several phytopathogenic fungi (Botrytis cinerea, Rhizoctonia solani and Pythium ultimum), in order to identify proteins of potential biotechnology value for commercial and industrial use. When T. harzianum proteins were analysed by two-dimensional electrophoresis (2-DE) substantial changes in the intracellular and extracellular proteomes of the mycoparasite were observed. Comparing the 2D maps of the fungus grown in minimal medium with glycerol as carbon source (used as control condition) with those obtained in inducing conditions, a lot of novel and up-regulated proteins appeared. Differentially expressed proteins were subjected to matrix assisted laser desorption ionisation-time of flight (MALDI-TOF) mass spectrometry (MS). The in silico analyses of some of the novel and up-regulated spots showed interesting homology to hypothetical and putative proteins from other fungal species. These include novel enzymes, such as glycosylhydrolases and metalloprotease, proteins with conserved domains involved in pathogen-host interactions, such as Ras that regulates signal transduction pathways or LRRs that is involved in host recognition, etc.. Work is in progress to demonstrate the role of some of these proteins in biocontrol and ability to induce systemic resistance.
Database assignments for author(s): Sheridan L. Woo, Francesco Vinale, Roberta Marra, Michelina Ruocco
Research topic(s) for pests/diseases/weeds:
biocontrol - natural enemies
Research topic(s) for beneficials or antagonists:
molecular biology - genes
Pest and/or beneficial records:
| Beneficial | Pest/Disease/Weed | Crop/Product | Country | Quarant.
|
|---|---|---|---|---|
| Botrytis cinerea | ||||
| Rhizoctonia solani | ||||
| Globisporangium ultimum | ||||
| Trichoderma harzianum (antagonist) | Botrytis cinerea | |||
| Trichoderma harzianum (antagonist) | Rhizoctonia solani | |||
| Trichoderma harzianum (antagonist) | Globisporangium ultimum |
