Journal of Plant Pathology (2004) 86, p. 296 (Greco et al.)

M. Greco and M. Scortichini (2004)
Phytotoxic and biocidal metabolites produced in culture by Pseudomonas avellanae
Journal of Plant Pathology 86 (4), 296-296
XI Meeting, Italian Society for Plant Pathology, Milan, 29/9 - 1/10, 2004
Abstract: Pseudomonas avellanae is the causal agent of bacterial canker and decline of hazelnut (Corylus avellana L.), which is characterized by an extensive withering of the trees during the vegetative season, which invariably leads to a rapid wilting (i.e. in one or two weeks). Two strains of the bacterium, from northern Greece and central Italy (province of Viterbo), were used to assess their in vitro capability to produce bioactive metabolites. After 11 days of growth at 16-25°C into still flasks containing the IMM medium with basic L-histidine instead of acid L-histidine, culture filtrates of both strains showed phytotoxic activity towards tobacco leaves (leaf necrosis and chlorosis), lemon fruits (necrosis and water-soaking) and bean pods (necrosis). Biocidal activity against Rhodotorula pilimanae, 600 units ml^-1, and Bacillus megaterium, 800 units ml^-1, was also observed. The metabolites contained in culture filtrates retained their activity after lyophilization, freezing at -80°C for three days, and heating at 50 and 80°C for 30 min. Shaken cultures had no phytotoxic activity. Likewise, no phytotoxic or biocidal activity was observed when cultures were grown in IMM medium with acid L-histidine, Woolley medium, potato-dextrose-casamino acid broth, and Gross minimal medium. The tested strains did not possess the syrB gene coding for the production of cyclic lipodepsinonapeptides such as the syringomycin toxin.
Database assignments for author(s): Marco Scortichini

Research topic(s) for pests/diseases/weeds:
molecular biology - genes

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Pest and/or beneficial records:

Beneficial	Pest/Disease/Weed	Crop/Product	Country	Quarant.
	Pseudomonas avellanae	Hazelnut (Corylus)	Greece
	Pseudomonas avellanae	Hazelnut (Corylus)	Italy