Journal of Plant Pathology (2004) 86, 337-338

M. Zaccardelli, M. Merighi, A. Spasiano and R. Torre (2004)
Detection of Xanthomonas campestris pv. campestris by PCR and PCR-RFLP analysis in symptomatic and symptomless plant material
Journal of Plant Pathology 86 (4), 337-338
XI Meeting, Italian Society for Plant Pathology, Milan, 29/9 - 1/10, 2004 (poster)
Abstract: Xanthomonas campestris pv. campestris (Pammel) Dowson (Xcc) is the causal agent of vascular blackening or black rot of crucifers. Symptoms consist of blackening of the vascular tissue and marginal chlorosis of the leaves. A rapid, reliable and specific PCR protocol for the identification of Xcc was recently optimised on pure cultures using two pathovar-specific oligonucleotide primers designed on the hrcC gene of the Hrp_Xcc pathogenicity island. The PCR analysis was performed on DNA extracted from naturally infected and artificially inoculated leaves of crucifers. In all cases the expected 519-bp amplicon was obtained. No amplicons were obtained from healthy plant material. The specificity of the diagnostic test performed on plant materials was improved by PCR-RFLP analysis of the amplicons with HaeIII.
(The abstract is excluded from the Creative Commons licence and has been copied with permission by the publisher.)
Database assignments for author(s): Massimo Zaccardelli

Research topic(s) for pests/diseases/weeds:
identification/taxonomy
surveys/sampling/distribution

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Pest and/or beneficial records:

Beneficial	Pest/Disease/Weed	Crop/Product	Country	Quarant.
	Xanthomonas campestris pv. campestris
	Xanthomonas campestris