Journal of Plant Pathology (2003) 85, 280-281

M. Pasquali, L. Marena, E. Fiora, P. Piatti, M.L. Gullino and A. Garibaldi (2003)
PCR real time identification of a highly pathogenic group of Fusarium oxysporum f.sp. chrysanthemi on Argyranthemum frutescens
Journal of Plant Pathology 85 (4), 280-281
X Meeting, Italian Society for Plant Pathology (oral presentation)
Abstract: A real time PCR system based on Taqman chemistry was developed in order to identify a new group of Fusarium oxysporum f.sp. chrysanthemi highly pathogenic to Paris daisy. Sensitivity of traditional PCR and real time PCR were compared on genomic DNA and on DNA extracted from infected plants with the use of a selective substrate. Real time PCR allowed the identification of infected plants on the fifth day after infection from stem tissue and on the second day from root tissue, even if the plants remained asymptomatic until the 13th day from infection. The sensitivity of real time PCR allowed the identification of 300 fg of genomic DNA. The possibility of automation of the process and the rapidity in the analysis is discussed and real time PCR is proposed as an elective system for diagnosis of this new epidemic disease.
(The abstract is excluded from the Creative Commons licence and has been copied with permission by the publisher.)
Database assignments for author(s): Matias Pasquali

Research topic(s) for pests/diseases/weeds:
identification/taxonomy

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Pest and/or beneficial records:

Beneficial	Pest/Disease/Weed	Crop/Product	Country	Quarant.
	Fusarium oxysporum f.sp. chrysanthemi
	Fusarium oxysporum