Journal of Phytopathology - Phytopathologische Zeitschrift (1997) 145, 235-238

From Pestinfo-Wiki
Jump to: navigation, search

A. Franck, G. Loebenstein and A. Gera (1997)
Use of the reverse transcription-polymerase chain reaction for the detection of pelargonium flower break carmovirus
Journal of Phytopathology - Phytopathologische Zeitschrift 145 (5-6), 235-238
Abstract: The specificity and sensitivity of RT-PCR were compared with the enzyme-linked immunosorbent assay (ELISA) for the detection of PFBV in Pelargonium tissues. The virus could be detected efficiently in high dilutions of sap from infected plants and at low concentrations of purified virus. Although ELISA is a powerful tool for virus detection, RT-PCR was over 1000 times more sensitive in detecting PFBV in leaf extracts of infected Pelargonium than was ELISA. The limit of detecting PFBV RNA by RT-PCR was 200 fg, compared with 200 pg of virus by ELISA.
(The abstract is excluded from the Creative Commons licence and has been copied with permission by the publisher.)
Link to article at publishers website
Database assignments for author(s): Gad Loebenstein, Abed Gera

Research topic(s) for pests/diseases/weeds:
identification/taxonomy
molecular biology - genes

Pest and/or beneficial records:

Beneficial Pest/Disease/Weed Crop/Product Country Quarant.


Alphacarmovirus pelargonii Pelargonium (crop)
Retrieved from "https://wiki.pestinfo.org/w/index.php?title=Journal_of_Phytopathology_-_Phytopathologische_Zeitschrift_(1997)_145,_235-238&oldid=590036"