Journal of Phytopathology (2012) 160, 496-499

Zhen Bi, Jinyun Li, Yanbin Guo, Hui Zheng, Bin Hu, Jianhui Wang and Huimin Wang (2012)
A plating-PCR technique for detection and quantification of the biological control agent Agrobacterium vitis strain E26 in soil under controlled conditions
Journal of Phytopathology 160 (9), 496-499
Abstract: Agrobacterium vitis strain E26 is a promising biocontrol agent of grapevine crown gall, an economically important disease of grape worldwide. In this report, we developed a Plating-PCR method that allows specific detection and quantification of E26 by combining classical microbiological techniques with molecular tools. Random amplified polymorphic DNA fingerprints were used to differentiate E26 from other A. vitis strains. A differentially amplified fragment from E26 was sequenced and characterized as a sequence characterized amplified region (SCAR) marker. Two primer pairs were then designed and evaluated for their specificity against E26. One of the two SCAR primer pairs, 740F/R, was further selected for specific detection of strain E26. A plating assay coupled to PCR with the SCAR primers 740F/R allowed the assessment of population dynamics of E26 in non-sterile grape rhizosphere soil under controlled conditions.
(The abstract is excluded from the Creative Commons licence and has been copied with permission by the publisher.)
Link to article at publishers website
Database assignments for author(s): Huimin Wang, Yanbin Guo

Research topic(s) for pests/diseases/weeds:
biocontrol - natural enemies
Research topic(s) for beneficials or antagonists:
identification/taxonomy

---

Pest and/or beneficial records:

Beneficial	Pest/Disease/Weed	Crop/Product	Country	Quarant.
	Allorhizobium vitis	Grapevine (Vitis)
Allorhizobium vitis (antagonist)	Allorhizobium vitis	Grapevine (Vitis)