Journal of Phytopathology (2011) 159, 582-584

Chong Huang, Zhenyu Sun, Jiahui Yan, Yong Luo, Haiguang Wang and Zhanhong Ma (2011)
Rapid and precise detection of latent infections of wheat stripe rust in wheat leaves using loop-mediated isothermal amplification
Journal of Phytopathology 159 (7-8), 582-584
Abstract: Wheat stripe rust caused by Puccinia striiformis f.sp. tritici (Pst) is one of the most important diseases of wheat worldwide. Detection of latent infection in leaves is critical for estimating the initial inoculum potential of epidemics. A loop-mediated isothermal amplification (LAMP) assay was developed and evaluated for the detection of Pst DNA in spores and wheat seedlings with latent infections. LAMP assay could detect as low as 2 pg/μl template DNA and detect latent infections from leaves as early as 24 h after inoculation. This provides a rapid and accurate method of estimating latent infection levels.
(The abstract is excluded from the Creative Commons licence and has been copied with permission by the publisher.)
Link to article at publishers website
Database assignments for author(s): Yong Luo, Zhanhong Ma, Haiguang Wang

Research topic(s) for pests/diseases/weeds:
identification/taxonomy
surveys/sampling/distribution

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Pest and/or beneficial records:

Beneficial	Pest/Disease/Weed	Crop/Product	Country	Quarant.
	Puccinia striiformis