Journal of Phytopathology (2010) 158, 691-697

Harun Bayraktar, Muharrem Türkkan and Fatma Sara Dolar (2010)
Characterization of Fusarium oxysporum f.sp. cepae from onion in Turkey based on vegetative compatibility and rDNA RFLP analysis
Journal of Phytopathology 158 (10), 691-697
Abstract: Seventy-five isolates of Fusarium oxysporum f.sp. cepae, the causal agent of basal plate rot on onion, were obtained from seven provinces of Turkey. The isolates were characterized by vegetative compatibility grouping (VCGs) and restriction fragment length polymorphism (RFLP) analysis of the nuclear ribosomal DNA intergenic spacer region (IGS). Forty-eight vegetative compatibility groups were found, each containing a single isolate. Only one isolate formed strong heterokaryons with the reference isolates of VCG 0423. Five isolates were heterokaryon self-incompatible. Restriction fragment analysis with six different enzymes revealed 13 IGS types among 75 F. oxysporum isolates from Turkey as well as 16 reference isolates from Colorado, USA. The majority of single-member VCGs produced identical RFLP banding patterns with minor deviations, considerably different from those of the reference VCG isolates. These results suggested that isolates of F. oxysporum f.sp. cepae in Turkey derived from distinct clonal lineages and mutations at one or more vegetative compatibility loci restrict heterokaryon formation.
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Link to article at publishers website
Database assignments for author(s): Fatma S. Dolar

Research topic(s) for pests/diseases/weeds:
molecular biology - genes

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Pest and/or beneficial records:

Beneficial	Pest/Disease/Weed	Crop/Product	Country	Quarant.
	Fusarium oxysporum f.sp. cepae		U.S.A. (SW)
	Fusarium oxysporum f.sp. cepae	Onion/garlic/leek (Allium)	Turkey
	Fusarium oxysporum