Journal of Phytopathology (2006) 154, 217-223

I. Fekih Hassen, S. Roussel, J. Kummert, H. Fakhfakh, M. Marrakchi and M.H. Jijakli (2006)
Development of a Rapid RT-PCR test for the detection of Peach latent mosaic viroid, Pear blister canker viroid, Hop stunt viroid and apple scar skin viroid in fruit trees from Tunisia
Journal of Phytopathology 154 (4), 217-223
Abstract: A reverse transcription polymerase chain reaction was developed to investigate the occurrence of 'peach latent mosaic viroid' (PLMVd), 'pear blister canker viroid' (PBCVd), 'hop stunt viroid' (HSVd) and 'apple scar skin viroid' (ASSVd) on fruit trees (peach, pear, almond and apple) in Tunisia. The test was initially performed with total RNA preparations from selected isolates and then applied to total RNA preparations from leaf or bark tissues of fruit trees collected in the north of Tunisia and the Sahel. PLMVd was found to occur in peach and pear trees, HSVd in pear, peach and almond trees, and PBCVd in pear trees. Mixed PBCVd-HSVd and PLMVd-HSVd infections occurred naturally in pear trees. ASSVd was not detected in any samples from apple trees. The identity of the detected viroids was confirmed by comparing their sequences with those of other previously characterized isolates. The test was then simplified by direct use of diluted crude plant extracts. The results obtained from crude sap extracts of leaves or bark tissues and from total RNA preparations were identical. This improved test is thus quick and useful for large-scale routine analysis. It can be used in a certification programme to contribute to prevention of the occurrence and spread of PLMVd, HSVd, PBCVd and ASSVd in Tunisia.
(The abstract is excluded from the Creative Commons licence and has been copied with permission by the publisher.)
Link to article at publishers website
Database assignments for author(s): M. Haissam Jijakli

Research topic(s) for pests/diseases/weeds:
identification/taxonomy
surveys/sampling/distribution

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Pest and/or beneficial records:

Beneficial	Pest/Disease/Weed	Crop/Product	Country	Quarant.
	Pear blister canker viroid	Pear (Pyrus)	Tunisia
	Hop stunt viroid	Almond (Prunus dulcis)	Tunisia
	Hop stunt viroid	Peach/nectarine (Prunus persica)	Tunisia
	Hop stunt viroid	Pear (Pyrus)	Tunisia
	Peach latent mosaic viroid	Peach/nectarine (Prunus persica)	Tunisia
	Peach latent mosaic viroid	Pear (Pyrus)	Tunisia