Journal of Pest Science (2018) 91, 919-926

Chengyu Chen, Peng Han, Weiyu Yan, Siyi Wang, Xueyan Shi, Xuguo Zhou, Nicolas Desneux and Xiwu Gao (2018)
Uptake of quercetin reduces larval sensitivity to lambda-cyhalothrin in Helicoverpa armigera
Journal of Pest Science 91 (2), 919-926
Abstract: Quercetin, one of the most abundant dietary flavonoids, is widely present in nature. In this study, the effects of quercetin ingestion on Helicoverpa armigera larvae growth and its sensitivity to lambda-cyhalothrin were assessed. The median lethal concentration (LC₅₀) value of lambda-cyhalothrin to quercetin-fed H. armigera larvae was 2.39-fold higher than the control, suggesting a reduced sensitivity to lambda-cyhalothrin. Piperonyl butoxide (PBO) treatment effectively synergized lambda-cyhalothrin efficacy in quercetin-fed H. armigera larvae with a synergistic ratio of 2.36. Moreover, the growth of H. armigera larvae was inhibited by the quercetin uptake. To understand the reduced sensitivity to lambda-cyhalothrin, we measured the enzymatic activity of cytochrome P450 monooxygenases (P450s), as well as transcriptional responses of three P450s genes in quercetin-fed H. armigera larvae. We found that cytochrome P450 monooxygenases involved in the observed lambda-cyhalothrin tolerance in quercetin-fed H. armigera. After fed on quercetin for 48 h, 7-ethoxycoumarin-O-deethylase (ECOD) activity was significantly elevated in quercetin-fed H. armigera larvae; meanwhile, the expression level of CYP6B6, CYP6B8 and CYP321A1 was up-regulated. In conclusion, P450s played a crucial role in the metabolic adaptation of H. armigera larvae to its host plant's secondary metabolites as well as synthetic insecticides. The reduced lambda-cyhalothrin sensitivity in quercetin-fed H. armigera larvae suggested that previous exposure to the host plant-derived quercetin is likely to compromise the efficacy of pyrethroid insecticides.
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Link to article at publishers website
Database assignments for author(s): Xi-Wu Gao, Nicolas Desneux, Xu-Guo Zhou

Research topic(s) for pests/diseases/weeds:
pesticide resistance of pest

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Pest and/or beneficial records:

Beneficial	Pest/Disease/Weed	Crop/Product	Country	Quarant.
	Helicoverpa armigera