Journal of Pest Science (2014) 87, 701-710

Rachel L.O. Olson, Roxanne E. Farris, Norman B. Barr and Anthony I. Cognato (2014)
Molecular identification of Trogoderma granarium (Coleoptera: Dermestidae) using the 16s gene
Journal of Pest Science 87 (4), 701-710
Abstract: The Khapra beetle, Trogoderma granarium Everts, is a global pest species of stored grain products. Accurate identification of Trogoderma specimens trapped within stores of grain is critical to surveillance and exclusion efforts for the Khapra beetle. To enhance diagnostic capacity, we report PCR-based diagnostic assays for Khapra beetle identification. Three methods such as conventional PCR, real-time PCR, and DNA sequencing are reported for the diagnosis of Trogoderma specimens captured within the U.S. All three methods discern the Khapra beetle based on variation in fragments of 16S mitochondrial DNA. To examine PCR assay stringency, ten native or introduced Trogoderma species and two Megatoma species were also examined. These DNA-based assays provide reliable identification of T. granarium regardless of condition, life stage, or taxonomic expertise of the investigator.
(The abstract is excluded from the Creative Commons licence and has been copied with permission by the publisher.)
Link to article at publishers website
Database assignments for author(s): Roxanne E. Farris

Research topic(s) for pests/diseases/weeds:
identification/taxonomy

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Pest and/or beneficial records:

Beneficial	Pest/Disease/Weed	Crop/Product	Country	Quarant.
	Trogoderma granarium
	Trogoderma glabrum
	Trogoderma variabile
	Trogoderma inclusum
	Trogoderma teukton