Journal of Nematology (2020) 52 (088)

M. Clara Vieira dos Santos, M. Teresa M. Almeida and Sofia R. Costa (2020)
First report of Meloidogyne naasi parasitizing turfgrass in Portugal
Journal of Nematology 52 (088)
Abstract: In an exploratory sampling of a football field in Porto, Portugal, the root-knot nematode, Meloidogyne naasi, previously unreported from the Iberian Peninsula, was detected. Diagnosis was based on the analysis of perineal patterns and esterase phenotypes of females excised from grass roots, morphometrics and molecular analysis (PCR with specific primers and analysis of partial 28S sequences obtained by amplification using the primers D2A/D3B) of second-stage juveniles (J2) extracted from soil. When collected in water, J2 aggregated into a worm-star. Endospores of Pasteuria penetrans were frequently found attached to the J2. To our knowledge, this is the first report of M. naasi in Portugal and in the Iberian Peninsula, and the first report of worm-star formation in Meloidogyne.
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Research topic(s) for pests/diseases/weeds:
surveys/sampling/distribution
biocontrol - natural enemies
Research topic(s) for beneficials or antagonists:
surveys/distribution/isolation

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Pest and/or beneficial records:

Beneficial	Pest/Disease/Weed	Crop/Product	Country	Quarant.
	Meloidogyne naasi		Portugal (continental)
Pasteuria penetrans (antagonist)	Meloidogyne naasi		Portugal (continental)