Journal of Nematology (2006) 38, p. 272 (Gao et al.)

X. Gao, A.L. Colgrove, K.N. Lambert and T.L. Niblack (2006)
Quantification of the soybean cyst nematode, Heterodera glycines, in soybean roots with real-time PCR
Journal of Nematology 38 (2), 272-272
Abstracts of Society of Nematologists 45th Annual Meeting, Lihue, Hawaii, 18-21 June 2006
Abstract: The soybean cyst nematode (SCN), Heterodera glycines, is a destructive pathogen of soybean, and causes significant yield losses worldwide. Recently, interest has increased in the evaluation of seed treatments with novel nematicidal compounds to protect young plants of soybean and other crops from nematode infections. Enumeration of nematodes in plant tissues, which is necessary for this type of evaluation, requires time-consuming staining procedures and labor-intensive microscopic observations. In this study, we explored the use of a real-time quantitative polymerase chain reaction (QPCR) assay based on detection of the chorismate mutase gene (Hg-cm^-1) for quantification of SCN. The QPCR protocol was sensitive and specific, and showed a high degree of correlation with the number of juveniles in roots. This assay will greatly reduce the amount of time and resources required to assess treatment effects on nematode infection in seedlings.
Database assignments for author(s): Xuebiao Gao, Kris N. Lambert, Terry L. Niblack

Research topic(s) for pests/diseases/weeds:
identification/taxonomy
surveys/sampling/distribution

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Pest and/or beneficial records:

Beneficial	Pest/Disease/Weed	Crop/Product	Country	Quarant.
	Heterodera glycines	Soybean (Glycine max)