Journal of Nematology (2006) 38, p. 268 (Colgrove et al.)

A.C. Colgrove, U. Reuter-Carlson and T.L. Niblack (2006)
A molecular and host range analysis of interspecific crosses of Heterodera species
Journal of Nematology 38 (2), 268-268
Abstracts of Society of Nematologists 45th Annual Meeting, Lihue, Hawaii, 18-21 June 2006
Abstract: Cyst nematode species in the schachtii group are considered closely related and interbreeding has been reported. 'Hybridization' of economically important species could have significance in field situations and laboratory research. Investigations of host-parasite interactions, such as host resistance to Heterodera glycines, could be aided by host-hybrid cyst nematode combinations involving model organisms (e.g., Arabidopsis, a poor host of H. glycines, but a known host of H. schachtii). A PCR-RFLP protocol that uses Fok1-digested ITS 1 fragments was developed to distinguish H. glycines and H. schachtii (Szalanski, et al., 1997, JON 29: 255-267). Fragments unique to each species included a 252-bp fragment for H. glycines and a 181-bp one for H. schachtii. Controlled crosses were used to develop Heterodera glycines-H. schachtii hybrids. Heterodera glycines-infected soybean (Glycine max) and H. schachtii-infected sugarbeet (Beta vulgaris) were placed in hydroponic culture 10 days after infestation (DAI). Virgin females were transferred from roots to agar plates at 25 DAL Males of the opposite species were added, and mating was observed. Egg-filled females were transferred to pots of Lespedeza striata 'Kobe', a common host. Cysts that developed on lespedeza roots were harvested and subjected to PCR-RFLP. The presence of both diagnostic bands confirmed hybrid progeny. In a separate test, cysts from isolates originally collected by George Bird from Michigan fields infested with both H. glycines and H. schachtii were subjected to marker and host range analysis. Preliminary results indicate that a mixture of genotypes, including naturally occurring hybrids, exist in these fields. Additional molecular and host range analyses are being conducted to further investigate controlled and natural hybridizations of H. glycines and H. schachtii.
Database assignments for author(s): Terry L. Niblack

Research topic(s) for pests/diseases/weeds:
general biology - morphology - evolution
molecular biology - genes

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Pest and/or beneficial records:

Beneficial	Pest/Disease/Weed	Crop/Product	Country	Quarant.
	Heterodera glycines
	Heterodera schachtii