Journal of Nematology (1994) 26, 596-598

P.A. Donald and T.L. Niblack (1994)
Distinguishing between viable and nonviable Heterodera glycines eggs
Journal of Nematology 26 (4S), 596-598
Abstract: Greenhouse tests were conducted to determine the persistence of nonviable Heterodera glycines eggs in soil. Laboratory and greenhouse tests were conducted to determine the usefulness of the vital stains acridine orange and tetrazolium red for differentiating viable and nonviable eggs of H. glycines. Nonviable (boiled) egg preparations were compared with preparations that were not boiled for their persistence in methyl bromide-fumigated soil. Boiled eggs persisted longer (>200 days) than untreated eggs, perhaps due to disinfestation of the egg suspensions by boiling. Neither stain was a good indicator of egg viability as inferred from infectivity of juveniles in a bioassay of the same egg preparations exposed to the stains.
(The abstract is excluded from the Creative Commons licence and has been copied with permission by the publisher.)
Full text of article
Database assignments for author(s): Terry L. Niblack

Research topic(s) for pests/diseases/weeds:
general biology - morphology - evolution
biocontrol - natural enemies
Research topic(s) for beneficials or antagonists:
general biology - morphology - evolution

---

Pest and/or beneficial records:

Beneficial	Pest/Disease/Weed	Crop/Product	Country	Quarant.
	Heterodera glycines