Journal of Medical Entomology (2006) 43, 1178-1184

Lizette L. Koekemoer, Luna Kamau, Claire Garros, Sylvie Manguin, Richard H. Hunt and Maureen Coetzee (2006)
Impact of the Rift Valley on restriction fragment length polymorphism typing of the major African malaria vector Anopheles funestus (Diptera: Culicidae)
Journal of Medical Entomology 43 (6), 1178-1184
Abstract: Anopheles funestus Giles (Diptera: Culicidae) is one of Africa's major malaria vectors. To understand population structure within An. funestus, various molecular markers have recently been developed. We investigated the impact of the Rift Valley on one such molecular marker, a polymerase chain reaction-restriction fragment length polymorphism (RFLP). This system distinguishes An. funestus populations into types M, W, and MW. Generally, these types correlate between geographical location and RFLP type, whereby M associates essentially with eastern Africa, W with western and central Africa, and MW with southern Africa. In total, 606 specimens were screened from 12 countries. Specimens from southern African localities were of the expected MW-type with the exception of the sample from Malawi, which showed all three types. The specimens from Tanzania showed the M- and MW-types, whereas specimens from Kenya showed M- and W-types. We also recorded different RFLP types not recorded previously. Type Y was recorded in Malawi, and Z-type was recorded from four localities: Angola, Malawi, Ghana, and Zambia.
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Database assignments for author(s): Lizette L. Koekemoer, Luna Kamau, Sylvie Manguin, Maureen Coetzee

Research topic(s) for pests/diseases/weeds:
molecular biology - genes
identification/taxonomy

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Pest and/or beneficial records:

Beneficial	Pest/Disease/Weed	Crop/Product	Country	Quarant.
	Anopheles funestus		Ghana
	Anopheles funestus		Kenya
	Anopheles funestus		Tanzania
	Anopheles funestus		Angola
	Anopheles funestus		Malawi
	Anopheles funestus		Zambia