Journal of Medical Entomology (2005) 42, 490-494

Ary Farajollahi, Wayne J. Crans, Patricia Bryant, Bruce Wolf, Kristen L. Burkhalter, Marvin S. Godsey, Stephen E. Aspen and Roger S. Nasci (2005)
Detection of West Nile viral RNA from an overwintering pool of Culex pipiens pipiens (Diptera: Culicidae) in New Jersey, 2003
Journal of Medical Entomology 42 (3), 490-494
Abstract: In total, 1,324 Culex pipiens pipiens L. female mosquitoes were collected at Ft. Hancock, Monmouth County, New Jersey, from January to March 2001-2003. Mosquitoes were held in an insectary at 27°C and a photoperiod of 16:8 (L:D) h for 6 to 21 d after which they were tested in 34 pools. West Nile viral RNA was detected in one pool by a TaqMan reverse transcription-polymerase chain reaction assay; however, infectious virus could not be isolated using either Vero cell plaque assay or C6/36 mosquito cells. Twenty females dissected in January and March 2003 confirmed ovarian diapause status. We suggest that the mode of infection in this pool of overwintering females may have been due to vertical (transgenerational) transmission.
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Database assignments for author(s): Ary Farajollahi

Research topic(s) for pests/diseases/weeds:
general biology - morphology - evolution

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Pest and/or beneficial records:

Beneficial	Pest/Disease/Weed	Crop/Product	Country	Quarant.
	Culex pipiens		U.S.A. (NE)