Journal of Medical Entomology (2001) 38, 462-464

David J. Dohm and Michael J. Turell (2001)
Effect of incubation at overwintering temperatures on the replication of west Nile virus in New York Culex pipiens (Diptera: Culicidae)
Journal of Medical Entomology 38 (3), 462-464
Abstract: We examined the effect of simulated overwintering temperatures on West Nile (WN) virus replication in Culex pipiens L. derived from mosquitoes collected during the autumn 1999 WN epizootic in New York. The WN virus was a strain isolated from a dead crow also collected during this outbreak. Virus was recovered from most mosquitoes held exclusively at 26°C. In contrast, none of the mosquitoes held exclusively at the lower temperatures had detectable infections. When mosquitoes were transferred to 26°C after being held at 10°C for 21–42 d, infection and dissemination rates increased with increased incubation at 26°C. Future studies involving the attempted isolation of WN virus from overwintering mosquitoes may benefit from holding the mosquitoes at 26°C before testing for infectious virus.
(The abstract is excluded from the Creative Commons licence and has been copied with permission by the publisher.)
Link to article at publishers website
Database assignments for author(s): David J. Dohm, Michael J. Turell

Research topic(s) for pests/diseases/weeds:
general biology - morphology - evolution
environment - cropping system/rotation

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Pest and/or beneficial records:

Beneficial	Pest/Disease/Weed	Crop/Product	Country	Quarant.
	Culex pipiens		U.S.A. (NE)