Journal of Medical Entomology (2000) 37, 802-806

Elvira Saraiva, Patrícia Fampa, Virna Cedeno, Max Bergoin, Eric Mialhe and Louis H. Miller (2000)
Expression of heterologous promoters in Lutzomyia longipalpis and Phlebotomus papatasi (Diptera: Psychodidae) cell lines
Journal of Medical Entomology 37 (6), 802-806
Abstract: To establish a transient expression system for genes introduced into sand fly cell lines, we tested the expression of the luciferase reporter gene under control of different promoters. Towards this end, we lipofected cell lines obtained from New and Old World sand flies, LL-5 from Lutzomyia longipalpis Lutz and Neiva and PP-9 from Phlebotomus papatasi Scopoli, respectively. The relative levels of luciferase expression were studied under control of Drosophila melanogaster Meigen heat shock protein 70 (hsp70), human cytomegalovirus, simian virus 40 or Junonia coenia (Hübner) densovirus (P9) promoters. The Drosophila heat shock protein 70 promoter, originating from insect genes, functioned as a strong promoter in both cell lines. Promoters from the different virus genes also were capable of driving transgene expression in both cell lines.
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Research topic(s) for pests/diseases/weeds:
general biology - morphology - evolution

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Pest and/or beneficial records:

Beneficial	Pest/Disease/Weed	Crop/Product	Country	Quarant.
	Phlebotomus papatasi
	Lutzomyia longipalpis