Journal of Medical Entomology (1994) 31, 496-499

Carlo Severini, Marino Marinucci and Michel Raymond (1994)
Insecticide resistance genes in Culex pipiens (Diptera: Culicidae) from Italy: Esterase B locus at the DNA level
Journal of Medical Entomology 31 (3), 496-499
Abstract: Restriction fragment length polymorphism (RFLP) analysis at the esterase B locus was performed in samples from four populations of Italian Culex pipiens pipiens L. to identify correctly, at the molecular level, the resistance gene. The absence of the amplified B2 haplotype was confirmed, indicating that it has not increased in frequency after its detection in continental Italy around 1985. The B4 haplotype was detected in all populations at the same frequencies as a previous starch gel survey. The possibility that B4 amplification has occurred only once and has migrated across western Mediterranean region is supported by the data. A1 also was detected indirectly by the presence of its closely linked B allele. The B5 haplotype apparently was absent. No amplification at the esterase B locus was detected, except B4, indicating a low rate of occurrence of new amplification at this locus.
(The abstract is excluded from the Creative Commons licence and has been copied with permission by the publisher.)

Research topic(s) for pests/diseases/weeds:
pesticide resistance of pest

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Pest and/or beneficial records:

Beneficial	Pest/Disease/Weed	Crop/Product	Country	Quarant.
	Culex pipiens		Italy