Journal of Invertebrate Pathology (1977) 29, 31-35

J. Kalmakoff, A.M. Crawford and S.G. Moore (1977)
A radioimmunoassay using labeled antibody for polyhedron protein from a nuclear polyhedrosis virus of Wiseana cervinata
Journal of Invertebrate Pathology 29 (1), 31-35
Abstract: A radioimmunoassay (RIA) for polyhedron protein based on precipitation of the antibody-antigen complex by sodium acetate buffer, pH 5.0, was studied. The effects of varying assay conditions and of using [³H]acetate- or ¹²⁵I-labeled antibodies were examined. The RIA could detect specific differences between the polyhedron protein from the nuclear polyhedrosis viruses of Bombyx mori and Wiseana cervinata at the level of 3 × 10⁴ polyhedral/ml when 125I-labeled antibodies were used. This procedure could provide the basis for making serological comparisons of polyhedron proteins and for detecting viruses in samples such as soil and bird feces.
(The abstract has been supplied by the author or ISPI and is excluded from the Creative Commons licence.)
(original language: English)
Link to article at publishers website
Database assignments for author(s): Allan M. Crawford

Research topic(s) for pests/diseases/weeds:
biocontrol - natural enemies
Research topic(s) for beneficials or antagonists:
identification/taxonomy
surveys/distribution/isolation

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Pest and/or beneficial records:

Beneficial	Pest/Disease/Weed	Crop/Product	Country	Quarant.
	Wiseana cervinata	Grasses/turf/rangeland	New Zealand
Alphabaculovirus wisignatae (entomopathogen)	Wiseana cervinata	Grasses/turf/rangeland	New Zealand