Journal of General Virology (2009) 90, 253-260

Omid Eini, S.A. Akbar Behjatnia, Satish Dogra, Ian B. Dry, John W. Randles and M. Ali Rezaian (2009)
Identification of sequence elements regulating promoter activity and replication of a monopartite begomovirus-associated DNA ß satellite
Journal of General Virology 90 (1), 253-260
Abstract: DNA ß is a circular single-stranded satellite DNA associated with certain monopartite begomoviruses (family Geminiviridae) which causes economically important diseases such as cotton leaf curl disease. DNA ß contains a single gene, ßC1, which encodes a pathogenicity protein responsible for symptom production. Transient expression studies in Nicotiana tabacum using the ß-glucuronidase reporter gene driven by a ßC1 promoter-deletion series of the DNA ß associated with cotton leaf curl Multan virus identified a 68 nt region (between -139 and -207) which is important for ßC1 transcription. This 68 nt region contains a G-box (CACGTG) located 143 nt upstream of the ßC1 start codon. Mutation of the G-box resulted in a significant reduction in ßC1 promoter activity and DNA ß replication efficiency. In addition, the G-box motif was found to bind specifically to a protein(s) in nuclear extracts prepared from tobacco leaf tissues. Our results indicate that interaction of the G-box motif with host nuclear factors is important for efficient gene expression and replication of DNA ß.
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Database assignments for author(s): John W. Randles, M. Ali Rezaian

Research topic(s) for pests/diseases/weeds:
molecular biology - genes

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Pest and/or beneficial records:

Beneficial	Pest/Disease/Weed	Crop/Product	Country	Quarant.
	Cotton leaf curl Multan virus