Journal of General Virology (2005) 86, 2101-2105

Michel Tribodet, Laurent Glais, Camille Kerlan and Emmanuel Jacquot (2005)
Characterization of Potato virus Y (PVY) molecular determinants involved in the vein necrosis symptom induced by PVY^N isolates in infected Nicotiana tabacum cv. Xanthi
Journal of General Virology 86 (7), 2101-2105
Abstract: Viral molecular determinant(s) involved in the tobacco vein necrosis (TVN) symptom induced by necrotic isolates of Potato virus Y (PVY) on Nicotiana tabacum cv. Xanthi leaves remain undetermined. Reference isolates belonging to PVY^N (infectious PVY^N-605 clone) and PVY^O (PVY^O-139) were used to produce PVY chimeric genomes by using reverse-genetic techniques. These chimeric clones were inoculated biolistically onto Nicotiana clevelandii plants to establish the clone, prior to being tested on N. tabacum for their ability to induce TVN symptoms. Comparison between sequence data and symptoms observed for each mutated PVY construct shows that the C-terminal part of the multifunctional HC-Pro protein includes two residues (K₄₀₀ and E₄₁₉) that are involved in TVN induced by PVY^N isolates. Site-directed mutagenesis was used to confirm that these two HC-Pro residues are involved in the TVN phenotype.
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Database assignments for author(s): Emmanuel Jacquot

Research topic(s) for pests/diseases/weeds:
general biology - morphology - evolution
molecular biology - genes

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Pest and/or beneficial records:

Beneficial	Pest/Disease/Weed	Crop/Product	Country	Quarant.
	Potato virus Y	Tobacco (Nicotiana tabacum)