Journal of General Virology (2002) 83, 2587-2591

María C. Vives, Luis Rubio, Luis Galipienso, Luis Navarro, Pedro Moreno and José Guerri (2002)
Low genetic variation between isolates of Citrus leaf blotch virus from different host species and of different geographical origins
Journal of General Virology 83 (10), 2587-2591
Abstract: The population structure and genetic diversity of Citrus leaf blotch virus (CLBV) were estimated by single-strand conformation polymorphism and nucleotide sequence analyses of two genomic regions located within the replicase (R) and the coat protein (C) genes. Analysis of 30 cDNA clones of each genomic region from two CLBV isolates showed that both isolates contained a predominant haplotype and others closely related. Analysis of 37 CLBV Spanish field isolates showed low genetic diversity (0·0041 and 0·0018 for genomic regions R and C, respectively). Comparison of 14 CLBV isolates from Spain, Japan, USA, France and Australia showed genetic diversities of 0·0318 (R) and 0·0209 (C), respectively. No correlation was found between genetic distance and geographical origin or host species of the isolates. The ratio between nonsynonymous and synonymous substitutions was the lowest found in a plant virus, indicating a strong negative selective pressure in both genomic regions.
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Database assignments for author(s): José Guerri, Luis Rubio

Research topic(s) for pests/diseases/weeds:
general biology - morphology - evolution
molecular biology - genes

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Pest and/or beneficial records:

Beneficial	Pest/Disease/Weed	Crop/Product	Country	Quarant.
	Citrus leaf blotch virus		France
	Citrus leaf blotch virus		Spain (continental)
	Citrus leaf blotch virus		Japan