Journal of General Virology (2000) 81, 1121-1126

Pierre-Yves Teycheney, Rachid Aaziz, Sylvie Dinant, Katalin Salánki, Colette Tourneur, Ervin Balázs, Mireille Jacquemond and Mark Tepfer (2000)
Synthesis of (-)-strand RNA from the 3' untranslated region of plant viral genomes expressed in transgenic plants upon infection with related viruses
Journal of General Virology 81 (4), 1121-1126
Abstract: When expressed in transgenic tobacco plants, transgene mRNA that includes the 3' untranslated region (3' UTR) of Lettuce mosaic virus served as template for synthesis of complementary (-)-strand RNA following an infection by Tobacco etch virus, Tobacco vein mottle virus or Pepper mottle virus, but not when infected with Cucumber mosaic virus. Deletion of the 3' UTR from the transgene abolished the synthesis of (-)-strand transcripts. Similar results were obtained in transgenic tobacco plants expressing mRNA that includes the RNA3 3' UTR of Cucumber mosaic virus when infected with Tomato aspermy virus. These results show that the viral RNA-dependent RNA polymerase of several potyviruses and Tomato aspermy virus have the ability to recognize heterologous 3' UTRs when included in transgene mRNAs, and to use them as transcription promoters.
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Database assignments for author(s): Pierre-Yves Teycheney, Mark Tepfer, Katalin Salanki, Mireille Jacquemond

Research topic(s) for pests/diseases/weeds:
molecular biology - genes

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Pest and/or beneficial records:

Beneficial	Pest/Disease/Weed	Crop/Product	Country	Quarant.
	Cucumber mosaic virus
	Pepper mottle virus
	Tobacco etch virus
	Tobacco vein mottling virus
	Lettuce mosaic virus
	Tomato aspermy virus