Journal of General Virology (1997) 78, 2701-2705

B. Martin, J.L. Collar, W.F. Tjallingii and A. Fereres (1997)
Intracellular ingestion and salivation by aphids may cause the acquisition and inoculation of non-persistently transmitted plant viruses
Journal of General Virology 78 (10), 2701-2705
Abstract: Transmission of non-persistent plant viruses is related to aphid behaviour during superficial brief probes. A widely accepted hypothesis postulates that virus acquisition occurs during ingestion of plant cell contents, and inoculation during egestion or regurgitation of previously ingested sap. Although conceptually attractive, this ingestion-egestion hypothesis has not been clearly demonstrated. Furthermore, it overlooks the anatomy of the tips of the stylets (mouthparts) and, consequently, the potential role of salivation in the inoculation process. Here, we used the electrical penetration graph (EPG) technique to investigate aphid-stylet activities associated with uptake (acquisition) and release (inoculation) of two non-persistently transmitted viruses. Our results show that acquisition occurs primarily during the last sub-phase (II-3) of intracellular stylet punctures, whereas inoculation is achieved during the first sub-phase (II-1). An alternative mechanism to the ingestion-egestion hypothesis is proposed on the basis of our findings.
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Database assignments for author(s): Alberto Fereres, W. Fred Tjallingii

Research topic(s) for pests/diseases/weeds:
transmission/dispersal of plant diseases

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Pest and/or beneficial records:

Beneficial	Pest/Disease/Weed	Crop/Product	Country	Quarant.
	Aphis gossypii	Melon (Cucumis melo)
	Myzus persicae	Green pepper/chilli (Capsicum)
	Potato virus Y	Green pepper/chilli (Capsicum)
	Cucumber mosaic virus	Melon (Cucumis melo)